Peptide reconstitution & dosage calculator.
This calculator turns four numbers you already have — how much peptide is in the vial, how much diluent you add, the dose you are studying, and your syringe — into the concentration, the volume and syringe units per dose, and how many doses the vial holds. It is arithmetic, nothing more: it never recommends doses and it is for research and education only.
As printed on the vial label or COA.
Bacteriostatic water you add to the vial.
A calculation input from your research reference — never a suggested dose.
Calibration is the barrel's marking scale — it stays fixed no matter which barrel you pick.
A 1 mL U-100 barrel holds 100 units but is still calibrated at 100 units per mL.
- Concentration
- 2.50 mg/mL · 2,500 mcg/mL
- Volume per dose
- 0.200 mL · 20.0 units
- Target dose
- 0.5 mg
- Doses per vial
- 10.0
About this peptide dosage calculator: enter your vial amount (mg), diluent volume, and target dose to get your reconstitution concentration in mg/mL and mcg/mL, the volume per dose in mL and syringe units (U-100, U-50, or U-40), and total doses per vial. Research calculation tool — it does not recommend doses or establish suitability for human use.
The math, shown.
Every result on this page comes from four arithmetic steps. Here is each formula, with the numbers worked for a 5 mg vial in 2 mL of diluent and a 500 mcg target dose on a U-100 syringe.
1. Concentration
concentration = peptide in vial ÷ diluent added
5 mg ÷ 2 mL = 2.5 mg/mL (= 2,500 mcg/mL)
2. Volume per dose
volume per dose = target dose ÷ concentration
500 mcg ÷ 2,500 mcg/mL = 0.2 mL
3. Syringe units
syringe units = volume × units per mL (calibration)
0.2 mL × 100 = 20 units on a U-100 syringe
4. Doses per vial
doses per vial = total peptide ÷ target dose
5,000 mcg ÷ 500 mcg = 10 doses
Units, explained.
The calculator only uses five ideas. Once these click, the results are easy to sanity-check yourself.
- mg and mcg
- Milligrams (mg) and micrograms (mcg) measure mass. 1 mg = 1,000 mcg, so a 5 mg vial holds 5,000 mcg. Peptide doses are usually discussed in mcg because the amounts are small.
- mL (milliliters)
- A milliliter measures liquid volume. The diluent you add (typically bacteriostatic water) is measured in mL, and every dose ends up expressed as a volume of liquid.
- Concentration (mg/mL)
- Concentration is how much peptide sits in each milliliter of liquid: peptide amount ÷ diluent volume. Doubling the diluent halves the concentration; the total peptide in the vial never changes.
- Syringe scale markings
- Insulin syringes are marked in units. On a U-100 syringe, every 10 units = 0.1 mL. On U-50, 10 units = 0.2 mL. On U-40, 10 units = 0.25 mL. The numbers on the barrel are volume in disguise.
- Calibration vs. capacity
- Calibration is the marking scale (U-100 = 100 units per mL). Capacity is how much the barrel physically holds. A 0.5 mL U-100 syringe only holds 50 units, but it is still calibrated at 100 units per mL — half a mL of liquid reads 50 units.
Related guides & calculators
Calculator FAQ.
How do I calculate peptide concentration after reconstitution?
Divide the total peptide amount in the vial by the diluent volume you add. For example, 5 mg of peptide in 2 mL of bacteriostatic water gives a concentration of 2.5 mg/mL.
How many doses are in one vial?
Divide the total amount of peptide by your target dose. A 5 mg vial holds ten 500 mcg doses, for example. The calculator shows this automatically.
What do U-100, U-50, and U-40 mean?
They are syringe calibrations — how many unit markings fit in one milliliter. U-100 = 100 units per mL, U-50 = 50 units per mL, U-40 = 40 units per mL. On a U-100 syringe, every 10 units is 0.1 mL.
Is a 50-unit syringe calibrated to 50 units per mL?
Not necessarily — that is the difference between capacity and calibration. A 0.5 mL U-100 syringe only holds 50 units, but its markings are still calibrated at 100 units per mL. The calculator keeps these separate: pick the calibration (the marking scale) and the barrel capacity (how much it holds) independently.
Why does my result show a red message or a dash?
The calculator refuses to compute with unusable values: a zero or negative vial amount, diluent volume, or dose, or a target dose larger than the total peptide in the vial. Fix the value in the red message and the results reappear.
How much bacteriostatic water should I add?
There is no single right amount — diluent volume only sets the concentration, and the total peptide in the vial never changes. More diluent means a weaker concentration and larger volumes per dose; less diluent means the opposite. Our reconstitution guide walks through the trade-offs.
Which syringe type should I select?
U-100 insulin syringes are the most common for research use. The calculator converts your dose volume into syringe units for U-100, U-50, and U-40 calibrations.
Is this calculator medical advice?
No — it is an educational tool for research use only. It performs arithmetic on numbers you enter; it does not suggest, recommend, or endorse any dose. Nothing on Exact Peptide is medical advice.